BUB1B, active, GST tagged human
SIGMA/SRP5327 - PRECISIO®, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
Synonym: BUB1beta; BUBR1; Bub1A; MAD3L; SSK1; hBUBR1
CAS Number: 203945-19-1
Product Type: Chemical
application(s) | cell analysis |
assay | ≥70% (SDS-PAGE) |
biological source | human |
form | buffered aqueous glycerol solution |
mol wt | ~73 kDa |
NCBI accession no. | NM_001211 |
product line | PRECISIO® |
recombinant | expressed in baculovirus infected Sf9 cells |
shipped in | dry ice |
specific activity | 1.2-1.8 nmol/min·mg |
storage temp. | −70°C |
General description: | BUB1B or budding uninhibited by benzimidazoles 1 homolog beta is involved in spindle checkpoint function and has been localized to the kinetochore. BUB1B plays a role in the inhibition of the anaphase-promoting complex/cyclosome (APC/C), delaying the onset of anaphase and ensuring proper chromosome segregation. BUB1B links the regulation of chromosome-spindle attachment to mitotic checkpoint signaling. BUB1B expression during the spindle checkpoint is dependent on residual BUB1B expression and impaired spindle checkpoint function has been found in many forms of cancer. BUB1B is essential for early embryonic development and normal hematopoiesis. |
Legal Information: | PRECISIO is a registered trademark of Sigma-Aldrich Co. LLC |
Physical form: | Supplied in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol. |
Preparation Note: | After opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles. |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 1 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Purity | ≥70% (SDS-PAGE) |
activity | specific activity: 1.2-1.8 nmol/min·mg |
Storage Temp. | −70°C |
UNSPSC | 12352200 |