NEK5, active, GST tagged human
SIGMA/SRP5328 - PRECISIO®, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
Synonym: MGC75495
CAS Number: 2249882-56-0
Product Type: Chemical
SDS-PAGE NEK5, active, GST tagged, human: Cat. No. SRP5328: Purity was determined by densitometry. Molecular weight ~84 kDa. See CofA for lot specific data.
Specific Activity NEK5, active, GST tagged, human: Cat. No. SRP5328: Specific Activity was determined by activity assay (see datasheet for protocol). See CofA for lot specific data.
| application(s) | cell analysis |
| assay | ≥70% (SDS-PAGE) |
| biological source | human |
| form | buffered aqueous glycerol solution |
| mol wt | ~84 kDa |
| NCBI accession no. | NM_199289   |
| product line | PRECISIO® |
| recombinant | expressed in baculovirus infected Sf9 cells |
| shipped in | dry ice |
| specific activity | 80-120 nmol/min·mg |
| storage temp. | −70°C |
| General description: | NEK5 or NIMA (never in mitosis gene a)-related kinase 5 is a serine/threonine kinase that is involved in cell cycle regulation. NEK5 has been shown to interact with NEK6 and NEK5 are required for the ibo1/nek6 ectopic outgrowth phenotype in epidermal cells. NEK6 interacts and co-localizes with NEK5 in a transient expression assay. The ibo1-3 mutation markedly increases the interaction between NEK6 and NEK5. NEK6 forms heterodimers with NEK5 to regulate cortical microtubule organization possibly through the phosphorylation of β-tubulins. The NEK5 gene is conserved in chimpanzee, cow, mouse, rat, and chicken. |
| Legal Information: | PRECISIO is a registered trademark of Sigma-Aldrich Co. LLC |
| Physical form: | Supplied in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol. |
| Preparation Note: | After opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥70% (SDS-PAGE) |
| activity | specific activity: 80-120 nmol/min·mg |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |