NEK1, active, GST tagged from mouse
SIGMA/SRP5329 - PRECISIO®, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
Synonym: D8Ertd790e; MGC189817; kat
Product Type: Chemical
SDS-PAGE NEK1, active, GST tagged, Mouse: Cat. No. SRP5329: Purity was determined by densitometry. Molecular weight ~85 kDa. See CofA for lot specific data.
Specific Activity NEK1, active, GST tagged, Mouse: Cat. No. SRP5329: Specific Activity was determined by activity assay (see datasheet for protocol). See CofA for lot specific data.
| assay | ≥70% (SDS-PAGE) |
| form | buffered aqueous glycerol solution |
| mol wt | ~85 kDa |
| NCBI accession no. | NM_175089   |
| product line | PRECISIO® |
| recombinant | expressed in baculovirus infected Sf9 cells |
| shipped in | dry ice |
| specific activity | 56-84 nmol/min·mg |
| storage temp. | −70°C |
| General description: | NEK1 or NIMA (never in mitosis gene a)-related kinase 1 is a serine/threonine kinase involved in cell cycle regulation and is found in a centrosomal complex with FEZ1, a neuronal protein that plays a role in axonal development. NEK1 is involved early in the DNA damage response pathway. NEK1 cycles through the nucleus via its nuclear localization and export signals. NEK1 protein participates in different signaling pathways to regulate diverse cellular processes and plays an important role in the kidney where it has opened a new avenue for studying cystogenesis and identifying possible modes of therapy. |
| Legal Information: | PRECISIO is a registered trademark of Sigma-Aldrich Co. LLC |
| Physical form: | Supplied in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol. |
| Preparation Note: | After opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥70% (SDS-PAGE) |
| activity | specific activity: 56-84 nmol/min·mg |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |