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DYRK3, active, GST tagged human

SIGMA/SRP5331 - PRECISIO®, recombinant, expressed in E. coli, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

Synonym: DYRK5; RED; REDK

CAS Number: 174206-56-5
Product Type: Chemical

Catalog Number PKG Qty. Price Quantity
45-SRP5331-10UG 10 µg
$518.00
1/EA
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SDS-PAGE DYRK3, active, GST tagged, human: Cat. No. SRP5331: Purity was determined by densitometry. Molecular weight ~95 kDa. See CofA for lot specific data.
Specific Activity DYRK3, active, GST tagged, human: Cat. No. SRP5331: Specific Activity was determined by activity assay (see datasheet for protocol). See CofA for lot specific data.

 

assay ≥70% (SDS-PAGE)
biological source human
form buffered aqueous glycerol solution
mol wt ~95 kDa
NCBI accession no. NM_001004023 
product line PRECISIO®
recombinant expressed in E. coli
shipped in dry ice
specific activity 194-290 nmol/min·mg
storage temp. −70°C
technique(s) cell culture | mammalian: suitable
General description: DYRK3 or dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 3 is belongs to the DYRK family of dual-specificity protein kinases that catalyze autophosphorylation on serine/threonine and tyrosine residues. DYRK3 expressed in E. coli undergoes tyrosine autophosphorylation and catalyzes phosphorylation of histones H3 and H2B in vitro. DYRK3 regulate different steps of the caveolar cycle. DYRK3 can promote cell survival through phosphorylation and activation of SIRT1. DYRK3 directly phosphorylate SIRT1 at Thr(522), promoting deacetylation of p53.
Legal Information: PRECISIO is a registered trademark of Sigma-Aldrich Co. LLC
Physical form: Supplied in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol.
Preparation Note: After opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles.
RIDADR NONH for all modes of transport
WGK Germany WGK 1
Flash Point(F) Not applicable
Flash Point(C) Not applicable
Purity ≥70% (SDS-PAGE)
activity specific activity: 194-290 nmol/min·mg
Storage Temp. −70°C
UNSPSC 12352200

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