GLK, active, GST tagged human
SIGMA/SRP5332 - PRECISIO®, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
Synonym: MAP4K3; MAPKKKK3; MEKKK3; RAB8IPL1
Product Type: Chemical
application(s) | cell analysis |
assay | ≥70% (SDS-PAGE) |
biological source | human |
form | buffered aqueous glycerol solution |
mol wt | ~70 kDa |
NCBI accession no. | NM_003618 |
product line | PRECISIO® |
recombinant | expressed in baculovirus infected Sf9 cells |
shipped in | dry ice |
specific activity | 74-110 nmol/min·mg |
storage temp. | −70°C |
General description: | GLK is a member of the STE20 family of serine/threonine protein kinases and contains N-terminal catalytic domain and C-terminal regulatory domain. GLK is upstream of the MEKK1 target and is stimulated by UV radiation and TNF-ï¡ and can lead to the activation of the JNK signaling pathway. GLK can directly activate PKC-θ during TCR signaling and people with systemic lupus erythematosus show considerable enhanced GLK expression and activation of PKC-θ and the kinase IKK in T cells. GLK can act as a pro-apoptotic kinase which orchestrates activation of BAX via the concerted posttranscriptional modulation of PUMA, BAD, and BIM. |
Legal Information: | PRECISIO is a registered trademark of Sigma-Aldrich Co. LLC |
Physical form: | Supplied in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol. |
Preparation Note: | After opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles. |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 1 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Purity | ≥70% (SDS-PAGE) |
activity | specific activity: 74-110 nmol/min·mg |
Storage Temp. | −70°C |
UNSPSC | 12352200 |