PP2C-α, active, GST tagged human
SIGMA/SRP5335 - recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
Synonym: FLJ42306; MGC9201; PP2C-ALPHA; PP2CA; PPM1A
CAS Number: 362690-38-8
Product Type: Chemical
SDS-PAGE PP2C-α, active, GST tagged, human: Cat. No. SRP5335: Purity was determined by densitometry. Molecular weight ~105 kDa. See CofA for lot specific data.
Specific Activity PP2C-α, active, GST tagged, human: Cat. No. SRP5335: Specific Activity was determined by activity assay (see datasheet for protocol). See CofA for lot specific data.
| application(s) | cell analysis |
| assay | ≥70% (SDS-PAGE) |
| biological source | human |
| form | buffered aqueous glycerol solution |
| mol wt | ~105 kDa |
| NCBI accession no. | NM_021003   |
| recombinant | expressed in baculovirus infected Sf9 cells |
| shipped in | dry ice |
| specific activity | 960-1440 nmol/min·mg |
| storage temp. | −70°C |
| Biochem/physiol Actions: | Protein phosphatase 2C-α (PP2C-α) or PPM1A is known to be negative regulators of cell stress response pathways. It has been reported to negatively regulate TGF-β signaling pathway and cell cycle. PP2C-α has been shown to dephosphorylate cyclin-dependent kinases (CDKs) and it may be involved in cell cycle control. It modulates cell migration and invasion. Overexpression of PP2C-α is reported to cause cell-growth arrest or cell death. |
| General description: | Protein phosphatase 2C-α (PP2C-α), also called protein phosphatase, Mg2+/Mn2+ dependent 1A (PPM1A), is a member of the family of Ser/Thr protein phosphatases and is composed of a catalytic subunit and a constant regulatory subunit. PP2C-α is highly expressed in heart and skeletal muscle. The gene encoding this protein is localized on human chromosome 4q12. |
| Physical form: | Supplied in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol. |
| Preparation Note: | After opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥70% (SDS-PAGE) |
| activity | specific activity: 960-1440 nmol/min·mg |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |