PDE1A, active, His tagged human
SIGMA/SRP5339 - recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
Synonym: HCAM1; HSPDE1A; MGC26303
Product Type: Chemical
SDS-PAGE PDE1A, active, His tagged, human: Cat. No. SRP5339: Purity was determined by densitometry. Molecular weight ~64 kDa. See CofA for lot specific data.
Specific Activity PDE1A, active, His tagged, human: Cat. No. SRP5339: Specific Activity was determined by activity assay (see datasheet for protocol). See CofA for lot specific data.
| assay | ≥70% (SDS-PAGE) |
| form | buffered aqueous glycerol solution |
| mol wt | ~64 kDa |
| NCBI accession no. | NM_005019   |
| recombinant | expressed in baculovirus infected Sf9 cells |
| shipped in | dry ice |
| specific activity | 10412-14134 nmol/min·mg |
| storage temp. | −70°C |
| General description: | PDE1A is a member of the cyclic nucleotide phosphodiesterase family that is stimulated by Ca2+-calmodulin. PDE1A is a cGMP-specific phosphodiesterase and hydrolyzes cGMP to 5′-GMP. Calmodulin is tightly bound to PDE1A in human spermatozoa and this results in permanent activation of PDE1A. Inhibition of PDE1A function significantly attenuates vascular smooth muscle cell growth by decreasing proliferation via G1 arrest and induces apoptosis. In vascular smooth muscle cell, PDE1A is important in regulating growth and survival of these cells and thus can contribute to the neointima formation in atherosclerosis and restenosis. |
| Physical form: | Supplied in 50 mM sodium phosphate, pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.2 mM DTT, 25% glycerol. |
| Preparation Note: | After opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles. |
| Symbol |   GHS07,GHS08 |
| Signal word | Danger |
| Hazard statements | H315 - H319 - H360D |
| Precautionary statements | P202 - P264 - P280 - P302 + P352 - P305 + P351 + P338 - P308 + P313 |
| Hazard Codes | T |
| Risk Statements | 61 |
| Safety Statements | 53-45 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥70% (SDS-PAGE) |
| activity | specific activity: 10412-14134 nmol/min·mg |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |