GST protein, tag-free
SIGMA/SRP5348 - recombinant, expressed in E. coli, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
CAS Number: 50812-37-8
Product Type: Chemical
SDS-PAGE GST protein, tag-free : Cat. No. SRP5348: Purity was determined by densitometry. Molecular weight ~25 kDa. See CofA for lot specific data.
| assay | ≥70% (SDS-PAGE) |
| form | buffered aqueous glycerol solution |
| mol wt | ~25 kDa |
| NCBI accession no. | U13850   |
| recombinant | expressed in E. coli |
| shipped in | dry ice |
| storage temp. | −70°C |
| technique(s) | ligand binding assay: suitable |
| Application: | GST protein, tag-free has been used in in vitro F-actin binding assay. It has also been used as a protein tag for TPX2 (targeting protein for Xklp2). |
| General description: | Glutathione S-transferase (GST) family of enzymes comprises a long list of cytosolic, mitochondrial and microsomal proteins that are used to create the so-called GST gene fusion system. These proteins are capable of multiple reactions with a multitude of substrates. They have a role in detoxication and toxification mechanisms. GST tag has the size of 220 amino acids, which is relatively large compared to other tags like the myc- or the FLAG-tag that are quite small. GST can be used to purify and detect proteins of interest. In a GST gene fusion system, an expression vector consists of the gene sequence encoding the protein of interest and GST sequence alongside. After induction, the fusion proteins can be purified using affinity chromatography via its high affinity for glutathione. |
| Physical form: | Supplied in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol. |
| Preparation Note: | After opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥70% (SDS-PAGE) |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |