IKK-γ, GST tagged human
SIGMA/SRP5349 - recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
Synonym: AMCBX1; FIP-3; FIP3; Fip3p; IKBKG; IKK-gamma; IP; IP1; IP2; IPD2; NEMO
CAS Number: 408328-74-5
Product Type: Chemical
SDS-PAGE IKK-γ, GST tagged, human: Cat. No. SRP5349: Purity was determined by densitometry. Molecular weight ~73 kDa. See CofA for lot specific data.
| application(s) | cell analysis |
| assay | ≥70% (SDS-PAGE) |
| biological source | human |
| form | buffered aqueous glycerol solution |
| mol wt | ~73 kDa |
| NCBI accession no. | NM_003639   |
| recombinant | expressed in baculovirus infected Sf9 cells |
| shipped in | dry ice |
| storage temp. | −70°C |
| General description: | IKKgamma is a serine/threonine protein kinase that phosphorylates the I-kappa-B protein which is an inhibitor of the transcription factor NF-kappa-B complex. Phosphorylation of I-kappa-B protein triggers the degradation of the inhibitor via the ubiquitination pathway, thereby activating NF-kappa-B complex. IKKgamma forms dimers and trimers and interacts preferentially with IKKbeta but not IKKalpha. IKKgamma associates with activated ATM after the induction of DNA double-strand breaks. ATM phosphorylates serine-85 of IKKgamma to promote its ubiquitin-dependent nuclear export. |
| Physical form: | Supplied in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol. |
| Preparation Note: | After opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥70% (SDS-PAGE) |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |