LMTK3, GST tagged from mouse
SIGMA/SRP5362 - recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
Synonym: AATYK3; MGC90743
Product Type: Chemical
assay | ≥70% (SDS-PAGE) |
form | buffered aqueous glycerol solution |
mol wt | ~75 kDa |
NCBI accession no. | NM_001005511 |
recombinant | expressed in baculovirus infected Sf9 cells |
shipped in | dry ice |
storage temp. | −70°C |
General description: | LMTK3, or Lemur tyrosine kinase 3, belongs to the LMR subfamily of tyr protein kinases. LMTK3 decreases the activity of protein kinase C (PKC) and also leads to phosphorylation of AKT (Ser473), thereby increasing binding of forkhead box O3 (FOXO3) to the ESR1 promoter. LMTK3 phosphorylates ER alpha, protecting it from proteasomal degradation in vitro. Knockdown of LMTK3 leads to a reduction of tumor volume in an orthotopic mouse model and abrogates proliferation of ER alpha+ but not ER alpha- cells, indicative of its role in ER alpha activity. These findings reveal LMTK3 as a new therapeutic target for breast cancer. |
Physical form: | Supplied in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol. |
Preparation Note: | After opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles. |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 1 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Purity | ≥70% (SDS-PAGE) |
Storage Temp. | −70°C |
UNSPSC | 12352200 |