VLK, GST tagged human
SIGMA/SRP5369 - recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
Synonym: PKDCC; SGK493
Product Type: Chemical
assay | ≥70% (SDS-PAGE) |
form | buffered aqueous glycerol solution |
mol wt | ~69 kDa |
NCBI accession no. | NM_138370 |
recombinant | expressed in baculovirus infected Sf9 cells |
shipped in | dry ice |
storage temp. | −70°C |
General description: | VLK or vertebrate lonesome kinase is a protein kinase which is first expressed in E-cadherin-positive anterior visceral endoderm and mesendoderm, and later its expression is confined to E-cadherin-negative mesenchyme. VLK regulates the rate of protein export from the Golgi and thereby plays an important role in the formation of functional stroma by mesenchymal cells. Targeted disruption of VLK leads to a defect in lung development and delayed ossification of endochondral bone. VLK deficient mice display neonatal lethality due to respiratory failure, with a suckling defect arising from a cleft palate. VLK is required for the appropriate timing of flat proliferative chondrocyte differentiation. |
Physical form: | Supplied in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol. |
Preparation Note: | After opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles. |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 1 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Purity | ≥70% (SDS-PAGE) |
Storage Temp. | −70°C |
UNSPSC | 12352200 |