INCENP, His tagged human
SIGMA/SRP5373 - recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
Synonym: FLJ31633; MGC111393
Product Type: Chemical
SDS-PAGE INCENP, His tagged, human: Cat. No. SRP5373: Purity was determined by densitometry. Molecular weight ~19 kDa. See CofA for lot specific data.
| assay | ≥70% (SDS-PAGE) |
| form | buffered aqueous glycerol solution |
| mol wt | ~19 kDa |
| NCBI accession no. | NM_001040694   |
| recombinant | expressed in baculovirus infected Sf9 cells |
| shipped in | dry ice |
| storage temp. | −70°C |
| Biochem/physiol Actions: | As part of the chromosomal passenger complex (CPC), inner centromere protein (INCENP) has a role in assembly and stability of the complex. It also aids in the centromere localization of CPC. INCENP has a role in mitosis and associates with microtubules. The protein has been shown to be upregulated in colorectal cancer cell lines. |
| General description: | INCENP or inner centromere protein antigens 135/155kDa is a constitutively binding centromere proteins and ′passenger,′ or transiently interacting, proteins. The overexpression of a truncation mutant of chicken INCENP and a chimeric CENPB:INCENP protein in mammalian cell culture disrupted both prometaphase chromosome alignment and completion of cytokinesis. INCENP forms a complex with the evolutionarily conserved family of Aurora B kinases which helps coordinate chromosome segregation, spindle behavior, and cytokinesis during mitosis. |
| Physical form: | Supplied in 50 mM sodium phosphate, pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.2 mM DTT, 25% glycerol. |
| Preparation Note: | After opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles. |
| Symbol |   GHS07,GHS08 |
| Signal word | Danger |
| Hazard statements | H315 - H319 - H360D |
| Precautionary statements | P202 - P264 - P280 - P302 + P352 - P305 + P351 + P338 - P308 + P313 |
| Hazard Codes | T |
| Risk Statements | 61 |
| Safety Statements | 53-45 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥70% (SDS-PAGE) |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |