Procathepsin K human
SIGMA/SRP6001 - recombinant, expressed in E. coli, ≥95% (SDS-PAGE)
Synonym: CTSK; CTSO; CTSO2
CAS Number: 177529-06-5
Product Type: Chemical
| assay | ≥95% (SDS-PAGE) |
| biological source | human |
| concentration | ~200 μg/mL |
| form | liquid |
| impurities | Endotoxin, tested |
| NCBI accession no. | P43235   |
| packaging | pkg of 10 μg |
| recombinant | expressed in E. coli |
| shipped in | dry ice |
| storage temp. | −70°C |
| Biochem/physiol Actions: | Cathepsin K has a role in bone resorption and remodeling. It has a triple helical collagen hydrolase activity. Mutations in the gene encoding this protein have been linked to pycnodysostosis. |
| General description: | Procathepsin K is the inactive proenzyme of cathepsin K. Cathepsin K is a lysosomal cysteine protease and part of the papain cysteine protease family. It is expressed in osteoclasts. The gene encoding this protein is localized on human chromosome 1q21. |
| Other Notes: | Procathepsin K can be activated by adjusting the pH 4.0 by adding an equal volume of 100 mM NaAc pH 3.9, 10 mM DTT, 5 mM EDTA followed by incubation for 40 min at RT. Activated mature Cathepsin is highly auto-proteolytic at pH 4.0, and care must be taken to avoid self-proteolysis. If the activated enzyme is not used immediately, we recommend to add methyl methanthiosulfonate (1 mM final concentration; MeS-SO2Me; MMTS) and to freeze the sample in liquid nitrogen or on dry ice. The hydrophobic thiol-reactive compound MMTS modifies cysteine’s by attaching its relatively small, uncharged thiomethyl-blocking group to reactive sulfhydryl groups (Nishimura et al., 1975). This reversible reaction arrests the auto-proteolytic process. The activity of the enzyme can be restored to nearly unmodified levels by adding L-cysteine (3M excess over MMTS) to the enzyme solution. |
| Physical form: | Liquid (25 mM Tris pH 8.0, 500 mM NaCl) |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥95% (SDS-PAGE) |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |