HGF human
SIGMA/SRP6014 - recombinant, expressed in CHO cells, ≥97% (SDS-PAGE), ≥97% (HPLC)
Synonym: F-TCF; HGF; HGFB; Hepatopoietin (HPTA); Scatter Factor (SF)
CAS Number: 953836-17-4
Product Type: Chemical
assay | ≥97% (HPLC) |
≥97% (SDS-PAGE) | |
biological source | human |
form | lyophilized |
impurities | Endotoxin, tested |
mol wt | 80.017 kDa |
NCBI accession no. | P14210 |
packaging | pkg of 1 mg |
pkg of 10 μg | |
recombinant | expressed in CHO cells |
shipped in | wet ice |
storage temp. | −20°C |
technique(s) | cell culture | embryo: suitable |
UniProt accession no. | P14210 |
Biochem/physiol Actions: | Hepatocyte growth factor (HGF) regulates both cell growth and cell motility. It exerts a strong mitogenic effect on hepatocytes and primary epithelial cells. HGF synergizes with interleukin-3 and granulocyte-macrophage colony-stimulating factor (GM-CSF) to stimulate colony formation of hematopoietic progenitor cells in vitro and may, therefore, also modulate hematopoiesis. It is expressed in brain tumors. |
General description: | Hepatocyte growth factor (HGF) is a multifunctional growth factor. HGF Human Recombinant produced in Chinese hamster ovary (CHO) is a heterodimer, non-glycosylated, polypeptide chain consisting of an α-chain of 463 amino acids and β-chain of 234 having a total molecular mass of 80.017kDa. The gene encoding this protein is localized on human chromosome 7q21.1. The HGF is purified by proprietary chromatographic techniques. |
Physical form: | The protein was lyophilized from a concentrated (1 mg/ml) solution containing PBS pH 7.4 containing 0.05% Tween 20 and 100 mM L-arginine. |
Reconstitution: | Centrifuge the vial prior to opening. It is recommended to reconstitute the lyophilized Hepatocyte Growth Factor (HGF) in sterile 18 MΩcm-1 H2O not less than 100 μg/mL, which can then be further diluted to other aqueous solutions. |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 3 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Purity | ≥97% (HPLC); ≥97% (SDS-PAGE) |
Storage Temp. | −20°C |
UNSPSC | 12352202 |