TIMP-1 human
SIGMA/SRP6040 - recombinant, expressed in Sf9 cells, ≥92% (SDS-PAGE)
Synonym: TIMP; TIMP recombinant; TIMP-1 protein; Tissue Inhibitor of matrix metalloproteinases-1
Product Type: Chemical
| assay | ≥92% (SDS-PAGE) |
| biological source | human |
| form | liquid |
| impurities | Endotoxin, tested |
| manufacturer/tradename | Croda International PLC |
| mol wt | 28.0 kDa |
| NCBI accession no. | P01033   |
| packaging | pkg of 5 μg |
| recombinant | expressed in Sf9 cells |
| shipped in | wet ice |
| storage temp. | −70°C |
| Biochem/physiol Actions: | TIMP1 functions as a poor inhibitor of MT1 (membrane type 1)-MMP, MT3-MMP, MT5-MMP and MMP-19. It inhibits ADAM10 (a disintegrin and metalloproteinase domain-containing protein 10) protein. In human umbilical vein endothelial cells (HUVECs), the down-regulation of TIMP1 and up-regulation of MMP-3 results in aberrant endothelium-dependent vasodilation, EC (endothelial cell) death, and endothelial interruption in a FOXO3 (forkhead box O3)-mediated manner. In patients with CAD (coronary artery disease) and acute coronary syndrome (ACS), the urine levels of this protein are elevated. This protein interacts with Bcl-2 (B cell lymphoma) protein, and induces apoptosis in lung adenocarcinoma cells. The plasma levels of this protein are increased in patients with obesity and obesity-related disorders, such as steatosis, where it participates in pathogenesis of diet-induced hepatic steatosis and glucose intolerance. |
| General description: | TIMP1 (tissue inhibitor of metalloproteinase 1) is a glycoprotein and is a member of TIMP family of endogenous MMP (matrix metalloproteinase) inhibitors. Humans contain four TIMPs. All TIMPs contain an N-terminal of 125 residues, a 65-residue C-terminal, and both these domains contain three disulfide bonds. The N-terminal domain inhibits MMPs by folding into a separate unit. TIMP1 is encoded by the gene mapped to human chromosome Xp11.23. |
| Legal Information: | Brij is a registered trademark of Croda International PLC |
| Physical form: | In 50 mM Tris-HCl, pH 7; 200 mM NaCl; 5 mM CaCl?; 1 ?M ZnCl?; 0.05% Brij® L23; 0,05% NaN? |
| Symbol |  GHS09 |
| Hazard statements | H411 |
| Precautionary statements | P273 - P391 - P501 |
| Hazard Codes | Xn,N |
| Risk Statements | 21/22-51/53 |
| Safety Statements | 36/37-61 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥92% (SDS-PAGE) |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |