ProBDNF human
SIGMA/SRP6053 - recombinant, expressed in E. coli, ≥95% (SDS-PAGE), ≥95% (HPLC)
Synonym: Abrineurin; BDNF; Brain-derived neurotrophic factor
Product Type: Product-on-demand
assay | ≥95% (HPLC) |
≥95% (SDS-PAGE) | |
biological source | human |
form | lyophilized |
mol wt | ~52 kDa |
NCBI accession no. | P23560 |
packaging | pkg of 10 μg |
recombinant | expressed in E. coli |
shipped in | wet ice |
storage temp. | −20°C |
Biochem/physiol Actions: | Precursor form of brain-derived neurotrophic factor (pro-BDNF) interacts preferentially with the pan-neurotrophin receptor p75 (p75NTR) (2) and vps10p domain-containing receptor sortilin and induces neuronal apoptosis, whereas mature BDNF selectively binds with high affinity to the tropomyosin-related kinase B (TrkB) kinase receptor and promotes the survival, growth and differentiation of neurons. As proneurotrophins and mature neurotrophins elicit opposite biological effects, pro-BDNF cleavage in the neuronal system is regulated in a specific and cell-context dependent manner. Pro-BDNF plays important role in negative regulation of neurotrophic actions in the brain. |
General description: | Brain-derived neurotrophic factor (BDNF) is a member of the neurotrophin family of growth factors. The precursor form of brain-derived neurotrophic factor (pro-BDNF) undergoes post-translational cleavage to form mature BDNF (mBDNF). The gene encoding this protein is localized on human chromosome 11p14.1. |
Other Notes: | Recombinant Human precursor form of Brain-derived neurotrophic factor produced in E. coli is a non-glycosylated polypeptide chain containing 2X229 amino acids as a homodimer and having a molecular mass of 52 kDa. The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Pro-Met-Lys-Glu. |
Physical form: | Lyophilized from a 0.2 μm filtered solution of 20 mM PB and 250 mM NaCl, pH 7.2. |
Preparation Note: | Centrifuge the vial prior to opening. |
Reconstitution: | Dissolve in 1x PBS (It is not recommended to reconstitute to a final concentration less than 100 μg/mL.). This can further be diluted to other aqueous buffers. |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 3 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Purity | ≥95% (HPLC); ≥95% (SDS-PAGE) |
Storage Temp. | −20°C |
UNSPSC | 12352202 |