G6PD from E. coli
SIGMA/SRP6112 - recombinant, expressed in E. coli, ≥90% (SDS-PAGE)
Synonym: Glucose-6-phosphate 1-dehydrogenase; Zwf
CAS Number: 9001-40-5
Product Type: Product-on-demand
Immunoblotting G6PD Cat. No. SRP6112: SDS-PAGE of human recombinant G6PD.
| assay | ≥90% (SDS-PAGE) |
| biological source | Escherichia coli |
| form | liquid |
| glucose-6-phosphate dehydrogenase activity | 8-10 U/mL (Specific activity is 8-10 units/ml obtained by measuring the increase of NADPH in absorbance at 340 nm resulting from the reduction of NAD or NADP.) |
| mol wt | 55.7 kDa (491 aa, 1-491 aa) |
| NCBI accession no. | P0AC53   |
| packaging | pkg of 100 μg |
| recombinant | expressed in E. coli |
| shipped in | dry ice |
| storage temp. | −70°C |
| UniProt accession no. | P0AC53 |
| Biochem/physiol Actions: | Glucose-6-phosphate dehydrogenase (G6PD) converts glucose-6-phosphate into 6-phosphoglucono-delta-la |
| General description: | Glucose-6-phosphate dehydrogenase (G6PD) is the rate-limiting enzyme of the pentose phosphate pathway, a metabolic pathway that supplies reducing energy to cells by maintaining the level of NADPH. |
| Other Notes: | MAVTQTAQAC DLVIFGAKGD LARRKLLPSL YQLEKAGQLN PDTRIIGVGR ADWDKAAYTK VVREALETFM KETIDEGLWD TLSARLDFCN LDVNDTAAFS RLGAMLDQKN RITINYFAMP PSTFGAICKG LGEAKLNAKP ARVVMEKPLG TSLATSQEIN DQVGEYFEEC QVYRIDHYLG KETVLNLLAL RFANSLFVNN WDNRTIDHVE ITVAEEVGIE GRWGYFDKAG QMRDMIQNHL LQILCMIAMS PPSDLSADSI RDEKVKVLKS LRRIDRSNVR EKTVRGQYTA GFAQGKKVPG YLEEEGANKS SNTETFVAIR VDIDNWRWAG VPFYLRTGKR LPTKCSEVVV YFKTPELNLF KESWQDLPQN KLTIRLQPDE GVDIQVLNKV PGLDHKHNLQ ITKLDLSYSE TFNQTHLADA YERLLLETMR GIQALFVRRD EVEEAWKWVD SITEAWAMDN DAPKPYQAGT WGPVASVAMI TRDGRSWNEF E |
| Physical form: | 1 mg/mL solution in 50 mM MES pH 6.0, 0.1 mM PMSF, 2 mM EDTA, 0.5 mM DTT and 10% glycerol. |
| Preparation Note: | Centrifuge the vial prior to opening. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥90% (SDS-PAGE) |
| activity | glucose-6-phosphate dehydrogenase activity: 8-10 U/mL (Specific activity is 8-10 units/ml obtained by measuring the increase of NADPH in absorbance at 340 nm resulting from the reduction of NAD or NADP.) |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |