DHFR from mouse
SIGMA/SRP6119 - recombinant, expressed in E. coli, ≥95% (SDS-PAGE)
Synonym: DYR; Dihydrofolate reductase; N/A
Product Type: Product-on-demand
Immunoblotting DHFR Cat. No. SRP6119: SDS-PAGE of mouse recombinant DHFR.
| assay | ≥95% (SDS-PAGE) |
| biological source | mouse |
| form | liquid |
| mol wt | 23.8 kDa (207 aa, 1-187 aa + NT His-Tag) |
| NCBI accession no. | P00375   |
| packaging | pkg of 100 μg |
| recombinant | expressed in E. coli |
| shipped in | dry ice |
| storage temp. | −70°C |
| UniProt accession no. | P00375 |
| Biochem/physiol Actions: | DHFR catalyzes the nicotinamide adenine dinucleotide phosphate (NADPH)-dependent reduction of dihydrofolate to tetrahydrofolate, and it is essential for the synthesis of thymidylate, purines and several amino acids. Expression of methotrexate (MTX)-resistant variants of DHFR in normal hematopoietic cells is a potential strategy to permit administration of larger doses of MTX by alleviating drug toxicity in normal cells and tissues that are drug sensitive. |
| General description: | Dihydrofolate reductase (DHFR) is a member of the reductase family of enzymes that is ubiquitously expressed in all organisms. The gene encoding this protein is localized on mouse chromosome 13. |
| Other Notes: | MGSSHHHHHH SSGLVPRGSH MVRPLNCIVA VSQNMGIGKN GDLPWPPLRN EFKYFQRMTT TSSVEGKQNL VIMGRKTWFS IPEKNRPLKD RINIVLSREL KEPPRGAHFL AKSLDDALRL IEQPELASKV DMVWIVGGSS VYQEAMNQPG HLRLFVTRIM QEFESDTFFP EIDLGKYKLL PEYPGVLSEV QEEKGIKYKF EVYEKKD |
| Physical form: | 1 mg/mL solution in 20 mM Tris-HCl buffer (pH 8.0) containing 0.1 M NaCl, 2 mM DTT and 10% glycerol. |
| Preparation Note: | Centrifuge the vial prior to opening. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H319 |
| Precautionary statements | P264 - P280 - P305 + P351 + P338 - P337 + P313 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥95% (SDS-PAGE) |
| Storage Temp. | −70°C |
| UNSPSC | 12352204 |