BMP-4 human
SIGMA/SRP6156 - recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)
Synonym: BMP-2B; Bone morphogenetic protein 2B; Bone morphogenetic protein 4
Product Type: Product-on-demand
TGF-beta Signaling
Research areas: Apoptosis, Differentiation, Epithelial Mesenchymal transition, Cancer biology, Bone remodelling, Developmental biology, Inflammation, Metabolism
Disease areas: Gastrointestinal cancers, Pulmonary, liver and renal fibrosis, Diabetes, Tuberculosis
See also: T7039; H8541; B3555; 11412272001; H4791; H8791
Research areas: Apoptosis, Differentiation, Epithelial Mesenchymal transition, Cancer biology, Bone remodelling, Developmental biology, Inflammation, Metabolism
Disease areas: Gastrointestinal cancers, Pulmonary, liver and renal fibrosis, Diabetes, Tuberculosis
See also: T7039; H8541; B3555; 11412272001; H4791; H8791
Immunoblotting BMP-4 Cat. No. SRP6156: SDS-PAGE of human cellexp human recombinant BMP-4.
| assay | ≥95% (SDS-PAGE) |
| biological source | human |
| form | lyophilized |
| impurities | <1 EU/μg protein Endotoxin level (LAL test) |
| mol wt | 34 kDa (homodimer, glycosylated.) |
| NCBI accession no. | P12644   |
| packaging | pkg of 10 μg |
| potency | 2-10 ng/mL |
| recombinant | expressed in HEK 293 cells |
| shipped in | dry ice |
| storage condition | avoid repeated freeze/thaw cycles |
| storage temp. | −20°C |
| Biochem/physiol Actions: | BMP4 (bone morphogenetic protein 4) is involved in modulating cell development in a wide variety of cells. It promotes the proliferation and migration of hepatocellular carcinoma cells, and the multiplication of ALK3 (activin-like kinase)-positive cells in skeletal muscle. This protein prevents the proliferation of lung fibroblasts and induces their differentiation into myocytes. In Sertoli cells, it promotes DNA synthesis and hence, the proliferation of Sertoli cells through Smad1/5 (small mothers against decapentaplegic) and ID2/3 (DNA binding protein inhibitor) pathway, and aberration in BMP4 expression results in azoospermia. In human males, the serum levels of BMP4 is linked with coronary artery disease (CAD) severity. BMP4 is crucial for maintaining the self-renewal capacity of ES (embryonic stem) cells, and achieves so by inhibiting extracellular receptor kinase (ERK) and p38 mitogen-activated protein kinase (MAPK) pathways. |
| General description: | BMP4 (bone morphogenetic protein 4), a member of the highly conserved BMP family, is a growth factor and a pro-inflammatory and pro-atherogenic cytokine. BMPs are part of the TGFβ (tumor growth factor) superfamily of proteins. BMP4 gene is localized to human chromosome 14q22-q23. |
| Physical form: | Lyophilized from a PBS solution. |
| Preparation Note: | Centrifuge the vial prior to opening. |
| Preparation Note: | Reconstitute in sterile 4 mM HCl containing 0.1% endotoxin-free recombinant human serum albumin. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥95% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |