Procathepsin K human
SIGMA/SRP6179 - recombinant, expressed in E. coli, ≥95% (SDS-PAGE)
Synonym: CTSK; CTSO; CTSO3
Product Type: Chemical
Immunoblotting Procathepsin K Cat. No. SRP6179, SDS-PAGE (15%) of hProcathepsin K. Lanes 1. Protein Marker 2. Human Procathepsin K (5 μg) 3. Human Procathepsin K (10 μg) 4. Human Procathepsin K (20 μg)
Immunoblotting Procathepsin K Cat. No. SRP6179, SDS-PAGE (15%) of activation of hProcathepsin K. Lanes 1. Protein Marker 2. Human Procathepsin K (5 μg) 3 to 7. Human Procathepsin K (5 μg) after 30 min, 1 h, 2 h, 3 h, and 4 h of activation
| assay | ≥95% (SDS-PAGE) |
| biological source | human |
| concentration | 1 mg/mL |
| form | liquid |
| mol wt | 35.9 kDa |
| packaging | pkg of 1 mg |
| pkg of 50 μg | |
| recombinant | expressed in E. coli |
| shipped in | dry ice |
| storage temp. | −70°C |
| UniProt accession no. | P43235   |
| General description: | Cathepsin K, a member of the papain cysteine proteinase family is the predominant proteinase responsible for the resorption of the bone matrix. Cathepsin cleaves proteins such as collagen type I, collagen type II and osteonectin, thereby playing a role in bone remodeling and resorption in osteoporosis, osteolytic bone metastasis and rheumatoid arthritis (Bromme and Okamoto, 1995; Drake, F. et al 1996; Bossard et al, 1996). Cathepsin K is synthesized as an inactive proenzyme (35.1 kDa) that is converted to its mature active form (23.6 kDa) by proteolytic cleavage of its 99-amino-acid propeptide domain. The in-vitro processing of procathepsin K to mature cathepsin K is autocatalytic. |
| Physical form: | 1 mg/mL solution in 25 mM Na2HPO4 and 500 mM NaCl (pH 7.0). |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥95% (SDS-PAGE) |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |