Procathepsin K from mouse
SIGMA/SRP6180 - recombinant, expressed in E. coli, ≥95% (SDS-PAGE)
Synonym: CTSK; CTSO; CTSO2; Cathepsin O; Cathepsin O2; Cathepsin X
Product Type: Chemical
Immunoblotting Procathepsin K, mouse Cat. No. SRP180, SDS-PAGE (17%) of purified Procathepsin K. Lanes 1. Protein marker 2. 10 μg of rm-Procathepsin K 3. 15 μg of rm-Procathepsin K 4. 20 μg of rm-Procathepsin K
Immunoblotting Procathepsin K, mouse Cat. No. SRP180, SDS-PAGE (17%) of Procathepsin K activated with 10 μg DTT. Lanes 1. Protein marker 2. rm-Procathepsin K after 4 h of activation with DTT (10 μg) 3. rm-Procathepsin K after 5 h of activation with DTT (10 μg) 4. rm-Procathepsin K after 7 h of activation with DTT (10 μg) 5. rm-Procathepsin K after 22 h of activation with DTT (10 μg)
| assay | ≥95% (SDS-PAGE) |
| biological source | mouse |
| concentration | 2.5 mg/mL |
| form | liquid |
| mol wt | 35.5 kDa |
| packaging | pkg of 1 mg |
| pkg of 10 μg | |
| pkg of 50 μg | |
| recombinant | expressed in E. coli |
| shipped in | dry ice |
| storage temp. | −70°C |
| UniProt accession no. | P43235   |
| General description: | Cathepsin K is a member of the papain cysteine proteinase family and has been identified as the predominant proteinase responsible for the resorption of the bone matrix. The enzyme cleaves proteins such as collagen type I, collagen type II and osteonectin and therefore plays a role in bone remodeling and resorption in diseases such as osteoporosis, osteolytic bone metastasis and rheumatoid arthritis (Bromme and Okamoto, 1995; Drake, F. et al 1996; Bossard et al, 1996). Cathepsin K is synthesized as an inactive proenzyme (35.1 kDa) that is converted to its mature active form (23.6 kDa) by proteolytic cleavage of its 99-amino-acid propeptide domain. The in-vitro processing of procathepsin K to mature cathepsin K is autocatalytic. |
| Physical form: | 2.5 mg/mL solution in 25 mM Na2HPO4 and 500 mM NaCl (pH 7.0). |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥95% (SDS-PAGE) |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |