Factor Va from human plasma
SIGMA/SRP6185 - ≥95% (SDS-PAGE)
Synonym: Activated protein C cofactor; Proaccelerin (labile factor)
Product Type: Chemical
Immunoblotting Factor Va, human Plasma Cat. No. SRP6185, Load: Human Factor Va, 1 μg/lane. Standard: Myosin (191 kDa), Phosphorylase B (97 kDa), BSA (64 kDa), Glutamic Dehydrogenase (51 kDa), Alcohol Dehydrogenase (39 kDa), Carbonic Anhydrase (28 kDa), Myoglobin Red (19 kDa), Lysozyme (14 kDa) Factor V is a very labile protein. Some degraded fragments are usually noticeable in preparations.
| assay | ≥95% (SDS-PAGE) |
| biological source | human plasma |
| form | liquid |
| mol wt | heavy chain 94.0 kDa |
| light chain 74 kDa | |
| packaging | pkg of 10 μg |
| shipped in | wet ice |
| storage temp. | −20°C |
| UniProt accession no. | P12259   |
| General description: | Factor Va is a cofactor for the serine protease factor Xa, and in the presence of calcium ions they collectively assemble on a phospholipid surface to form the prothrombinase complex. The prothrombinase complex is responsible for the rapid conversion of prothrombin to thrombin. Factor Va is derived from the pro-cofactor, factor V, upon limited proteolysis by α-thrombin. The thrombin cleavage of factor V liberates two heavily glycosylated activation peptides from the central portion of the molecule which have no cofactor function. Factor Va is comprised of an NH2-terminal derived heavy chain (Mr=94,000) and a COOH-terminal derived light chain (Mr=74,000) which remain associated in the presence of calcium ions. The cofactor binds to phospholipid (cell membrane) surfaces and effectively serves as a receptor for membrane bound factor Xa. Complete assembly of the prothrombinase complex (factor Xa, factor Va, phospholipid, and calcium) results in a 300,000-fold increase in the rate of prothrombin conversion relative to the rate observed with factor Xa alone. The interaction between factor Va and factor Xa is mediated by both the heavy and light chain of factor Va, while the binding of prothrombin to factor Va is mediated solely by the heavy chain. |
| Physical form: | liquid in 41.3 μl 20 mM HEPES, 150 mM NaC1, pH 7.5, 5 mM CaCl2 +50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥95% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |