Leptin Receptor human
SIGMA/SRP6192 - recombinant, expressed in E. coli, ≥95% (SDS-PAGE)
Synonym: CD_antigen=CD295; HuB219; OB Protein; OB receptor; OBS; Obesity Protein; Obesity factor
Product Type: Chemical
Immunoblotting Leptin Receptor Cat. No. SRP6192, Recombinant Human Leptin Receptor / LEPR.
| assay | ≥95% (SDS-PAGE) |
| biological source | human |
| form | lyophilized |
| mol wt | 95 kDa (predicted 130-140 kDa on a gel.) |
| packaging | pkg of 1 mg |
| pkg of 10 μg | |
| pkg of 50 μg | |
| recombinant | expressed in E. coli |
| shipped in | wet ice |
| storage temp. | −70°C |
| UniProt accession no. | P41159   |
| General description: | Leptin receptor (LEPR), also known as OB-R and B219, is a single transmembrane-domain receptor of the cytokine receptor family. Leptin receptor exists as homodimer and binds Leptin with high affinity, thus mediates the biological function of the adipocyte-specific hormone Leptin. LEPR is expressed at high levels in hematopoietic stem cells, lymphohematopoietic cell lines, as well as adult reproductive organs. Several isoforms of LEPR have been identified, and LEPR structurally contains two hemopoietin receptor domains, a fibronectin type III domain and a WSXWS domain within the extracellular region. Interaction of leptin and leptin receptor is crucial for bodyweight and bone mass regulation in mammals through hypothalamic effects on satiety and energy expenditure. Meanwhile, research data supports a leptin receptor activation model based on ligand-induced conformational changes. |
| Physical form: | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. |
| Reconstitution: | Centrifuge the vial prior to opening. The lyophilized Leptin Binding Domain is very soluble in H₂O and most aqueous buffers below and above the isoelectric point (~ 5.9). |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥95% (SDS-PAGE) |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |