Neuropoietin murine
SIGMA/SRP6228 - recombinant, expressed in E. coli, ≥98% (SDS-PAGE)
Synonym: NP; NPO
Product Type: Chemical
| assay | ≥98% (SDS-PAGE) |
| biological source | mouse |
| form | lyophilized powder |
| impurities | <1 EU/μg endotoxin (LAL test) |
| mol wt | 19.8 kDa |
| packaging | pkg of 10 and 50 μg |
| recombinant | expressed in E. coli |
| shipped in | wet ice |
| storage temp. | −20°C |
| UniProt accession no. | P83714   |
| General description: | Neuropoietin is a newly identified member of the IL-6 cytokine family. Members of this family, including IL-6, IL-11, oncostatin M, leukemia inhibitory factor (LIF), cardiotrophin-1 (CT-1), cardiotrophin-like cytokine, and CNTF, display a four-helix bundle structure, and signal through gp130-containing receptor complexes. Neuropoietin, which is predominantly expressed in neuroepithelia during embryonic life, acts through a receptor complex comprising CNTF receptor-α component, gp130, and LIF receptor. Like CNTF, it promotes the survival of embryonic motor neurons and could increase the proliferation of neural precursor cells in the presence of EGF and FGF-2. Interestingly, the human Neuropoietin gene has evolved toward a pseudogene, suggesting that the alternative signaling via CNTF is an effective compensatory pathway. Recombinant murine Neuropoietin is a 19.8 kDa protein containing 183 amino acid residues. |
| Physical form: | Sterile filtered through a 0.2 micron filter. Lyophilized from 2.5 mM Tris, pH 10.2 and 0.5 mM DTT. |
| Reconstitution: | Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H319 |
| Precautionary statements | P264 - P280 - P305 + P351 + P338 - P337 + P313 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥98% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |