SPARC/Osteonectin human
SIGMA/SRP6237 - recombinant, expressed in CHO cells, ≥97% (SDS-PAGE)
Synonym: BM-40; ON; Secreted protein acidic and rich in cysteine
Product Type: Chemical
assay | ≥97% (SDS-PAGE) |
biological source | human |
form | lyophilized powder |
impurities | <1 EU/μg endotoxin (LAL test) |
mol wt | 43.7 kDa |
packaging | pkg of 10 μg |
pkg of 50 μg | |
recombinant | expressed in CHO cells |
shipped in | wet ice |
storage temp. | −20°C |
UniProt accession no. | P09486 |
General description: | SPARC/Osteonectin is a secreted, evolutionarily conserved collagen-binding glycoprotein that is involved in a variety of cellular activities. It is highly expressed in tissues undergoing morphogenesis, remodeling and wound repair. SPARC/Osteonectin and its related peptides bind to numerous proteins of the extracellular matrix (ECM), affect ECM protein expression, influence cellular adhesion and migration, and modulate growth factor-induced cell proliferation and angiogenesis. SPARC/Osteonectin consists of three domains; an N-terminal acidic region that binds calcium ions with low affinity, a module containing two EF-hand motifs that bind calcium with high affinity, and a cysteine-rich follistatin-like domain. Recombinant human SPARC/Osteonectin is a glycoprotein containing 286 amino acids that migrates at an apparent MW of 43.7 kDa by SDSPAGE analysis due to the effect of glycosylation |
Physical form: | Sterile filtered through a 0.2 micron filter. Lyophilized from 10 mM Sodium Phosphate, pH 7.6. |
Reconstitution: | Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C. |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 1 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Purity | ≥97% (SDS-PAGE) |
Storage Temp. | −20°C |
UNSPSC | 12352200 |