VAP-1 human
SIGMA/SRP6241 - recombinant, expressed in CHO cells, ≥95% (SDS-PAGE)
Synonym: AOC3; Copper Amine Oxidase; HPAO; Membrane Primary Amine Oxidase; SSAO; Vascular Adhesion Protein-1
Product Type: Chemical
| assay | ≥95% (SDS-PAGE) |
| biological source | human |
| form | lyophilized powder |
| impurities | <1 EU/μg endotoxin (LAL test) |
| mol wt | ~80 kDa |
| packaging | pkg of 10 μg |
| pkg of 50 μg | |
| recombinant | expressed in CHO cells |
| shipped in | wet ice |
| storage temp. | −20°C |
| UniProt accession no. | Q16853   |
| Biochem/physiol Actions: | VAP-1 (vascular adhesion protein 1) catalyzes an oxygen-dependent reaction involving the conversion of primary amines to aldehyde, ammonia and hydrogen peroxide. Overexpression of VAP-1 is observed in chronic inflammatory diseases such as chronic renal failure, diabetesand autoimmune disorders. VAP-1 mediates the interaction between leukocytes and endothelium in response to inflammation. |
| General description: | VAP-1 (vascular adhesion protein 1) is also called as semicarbazide-sensitive amine oxidase. The VAP-1 gene is mapped to human chromosome 17q21.31. |
| General description: | VAP-1 is a type II membrane cell adhesion protein belonging to the copper/topaquinone oxidase family. It is primarily expressed on the high endothelial venules of peripheral lymph nodes and on hepatic endothelia. VAP-1 can catalyze the oxidative deamination of low molecular weight amines, and plays an important role in the migration of lymphocytes to inflamed tissue. Inhibition of VAP-1 can protect against inflammation related damage to certain injured tissues. Additionally, VAP-1 can function as a significant prognostic marker for certain cancers and cardiovascular diseases. Recombinant VAP-1 is a mixture of monomeric and disulfide linked homodimeric forms of a 737 amino acid polypeptide corresponding to amino acids 27 to 763 of the VAP-1 precursor. |
| Physical form: | Sterile filtered through a 0.2 micron filter. Lyophilized from 10 mM Sodium Phosphate, pH 7.8. |
| Reconstitution: | Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥95% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |