PLGF/PIGF2/PGF human
SIGMA/SRP6263 - recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)
Synonym: PGFL; SHGC-10760
Product Type: Chemical
assay | ≥95% (SDS-PAGE) |
biological source | human |
form | lyophilized powder |
impurities | <1 EU/μg endotoxin (LAL test) |
mol wt | calculated mol wt 20.2 kDa |
observed mol wt 28-33 kDa (DTT-reduced. Protein migrates due to glycosylation. Arg 29 is the predicted N-terminus.) | |
packaging | pkg of 10 μg |
recombinant | expressed in HEK 293 cells |
shipped in | wet ice |
storage temp. | −20°C |
tag | 6-His tagged (C-terminus) |
UniProt accession no. | P49763 |
Biochem/physiol Actions: | PLGF might enhance the production of peripheral blood mononuclear cell-vascular endothelial growth factor (PBMC-VEGF). Consequently, PlGF might facilitate VEGF production in the inflammatory joint. Soluble fms related tyrosine kinase 1 (Flt-1), which is considered to be an anti-angiogenic factor interacts with and inhibit the activity of PLGF. Therefore, PLGF level is lowered and the Flt-1 level is increased in placental tissue of women with pre-eclampsia (PE). PLGF is associated with the pathogenesis of rheumatoid arthritis (RA) and various other arthropathies. PLGF acts as a ligand for VEGFR-1 and is implicated in various physiological functions such as tumor angiogenesis, wound healing and hematopoietic progenitors recruitment. |
General description: | Placental growth factor (PLGF) exists in two isoforms namely PLGF1 and PLGF2. This protein is a homodimeric glycoprotein with molecular weight of 46–50kDa and is a member of the VEGF (vascular endothelial growth factor) subfamily. PLGF is encoded by the gene mapped to human chromosome 14q24-q31 regions. PLGF is expressed mainly in the placental trophoblast during pregnancy and is also present in various other tissues such as the villous trophoblast. |
Physical form: | Lyophilized from 0.22 μm filtered solution in PBS. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization. |
Reconstitution: | Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C. |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 3 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Purity | ≥95% (SDS-PAGE) |
Storage Temp. | −20°C |
UNSPSC | 12352200 |