MMP-2 human

SIGMA/SRP6270 - recombinant, expressed in HEK 293 cells, ≥92% (SDS-PAGE)

Synonym: CLG4; CLG4A; MMP-II; MMP2; MONA; TBE-1

Product Type: Chemical

Catalog Number	PKG Qty.	Price	Quantity


45-SRP6270-10UG	10 µg	$381.00 1/EA		Add To Favorites
45-SRP6270-50UG	50 µg	$1460.00 1/EA		Add To Favorites

Immunoblotting MMP-2 Cat. No. SRP6270, Human recombinant MMP-2.

Properties
Descriptions
Safety Info.
Basic Data

assay	≥92% (SDS-PAGE)
biological source	human
form	lyophilized powder
impurities	<1 EU/μg endotoxin (LAL test)
mol wt	calculated mol wt 71.8 kDa
	observed mol wt 66-71 kDa (DTT-reduced. Ala 30 is the predicted N-terminus.)
packaging	pkg of 10 and 50 μg
recombinant	expressed in HEK 293 cells
shipped in	wet ice
storage temp.	−20°C
tag	6-His tagged (C-terminus)
UniProt accession no.	P08253

Biochem/physiol Actions:	Matrix metalloproteinase-2 (MMP-2) cleaves the collagen-like sequence Pro-Gln-Gly-\|-Ile-Ala-Gly-Gln. It is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. It degrades type IV, V, VII, and X collagens. The protein degrades extracellular matrix proteins and acts on several non-matrix proteins such as big endothelial 1 (bET) and β-type calcitonin gene-related peptide (CGRP) promoting vasoconstriction. MMP-2 also cleaves KISS (metastasis suppressor) at a Gly-\|-Leu bond. The protein appears to have a role in myocardial cell death pathways and contributes to myocardial oxidative stress by regulating the activity of glycogen synthase kinase-3 β (GSK3β). It has been shown to cleave GSK3β in vitro. PEX, the C-terminal non-catalytic fragment of MMP2, possesses anti-angiogenic and anti-tumor properties and inhibits cell migration and cell adhesion to fibroblast growth factor-2 (FGF2) and vitronectin.
General description:	Matrix metalloproteinase-2 (MMP-2) which is also known as 72kDa type IV collagenase, 72kDa gelatinase, gelatinase A and CLG4A, belongs to the peptidase M10A family. It contains three fibronectin type-II domains and four hemopexin-like domains. MMP-2 is produced by normal skin fibroblasts. The gene encoding this protein is localized on human chromosome 16q12.2.
Physical form:	Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH 8.0. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.
Reconstitution:	Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Purity	≥92% (SDS-PAGE)
Storage Temp.	−20°C
UNSPSC	12352200

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