PCSK9 human
SIGMA/SRP6285 - recombinant, expressed in HEK 293 cells, ≥97% (SDS-PAGE)
Synonym: FNDC5 (cleaved); Fibronectin Type III Domain-containing Protein 5 (cleaved); Fibronectin Type III Repeat-containing Protein 2 (cleaved)
Product Type: Chemical
Immunoblotting PCSK9 Cat. No. SRP6285, Human Recombinant PCSK9.
| assay | ≥97% (SDS-PAGE) |
| biological source | human |
| form | lyophilized powder |
| impurities | <1 EU/μg endotoxin (LAL test) |
| mol wt | calculated mol wt 75.1 kDa |
| observed mol wt 20 kDa (DTT-reduced. Protein migrates due to glycosylation and proteolytic digestion.Gln 31 is the predicted N-terminus.) | |
| observed mol wt 62 kDa | |
| packaging | pkg of 20 μg |
| recombinant | expressed in HEK 293 cells |
| shipped in | wet ice |
| storage temp. | −20°C |
| tag | 6-His tagged (C-terminus) |
| UniProt accession no. | Q8NBP7   |
| General description: | Proprotein convertase subtilisin/kexin type 9 (PCSK9), is an enzyme which in humans is encoded by the PCSK9 gene. This gene encodes a proprotein convertase belonging to the proteinase K subfamily of the secretory subtilase family. This protein plays a major regulatory role in cholesterol homeostasis. PCSK9 binds to the epidermal growth factor-like repeat A (EGF-A) domain of the low-density lipoprotein receptor (LDLR), inducing LDLR degradation. PCSK9 may also have a role in the differentiation of cortical neurons. Mutations in this gene have been associated with a rare form of autosomal dominant familial hypercholesterolemia (HCHOLA3). |
| Physical form: | Sterile filtered through a 0.22 micron filter. Lyophilized from 1 x PBS, pH 7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization. |
| Reconstitution: | Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 100 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Purity | ≥97% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |