GFER/HPO/ALR human
SIGMA/SRP6289 - recombinant, expressed in HEK 293 cells, ≥92% (SDS-PAGE)
Synonym: ERV1; HERV1; HPO1; HPO2; HSS; Hepatopoietin
Product Type: Chemical
Immunoblotting GFER/HPO/ALR Cat. No. SRP6289, Human Recombinant GFER/HPO/ALR.
| assay | ≥92% (SDS-PAGE) |
| biological source | human |
| form | lyophilized powder |
| impurities | <1 EU/μg endotoxin (LAL test) |
| mol wt | calculated mol wt 15.2 kDa (no tag) |
| observed mol wt 16.0 kDa (DTT-reduced. Met 81 is the predicted N-terminus .) | |
| packaging | pkg of 10 μg |
| recombinant | expressed in HEK 293 cells |
| shipped in | wet ice |
| storage temp. | −20°C |
| UniProt accession no. | P55789   |
| Biochem/physiol Actions: | Growth factor, augmenter of liver regeneration (GFER) controls mitochondrial fission and apoptosis in liver ischemia. Isoform 1 of GFER regenerates the redox-active disulfide bonds in CHCHD4/MIA40, a chaperone essential for disulfide bond formation and protein folding in the mitochondrial intermembrane space. The reduced form of coiled-coil-helix-coiled- |
| General description: | Growth factor, augmenter of liver regeneration (GFER) is also known as FAD-linked sulfhydryl oxidase ALR, which belongs to the Erv1/ALR family of proteins. This family can be found in higher and lower eukaryotes. There are two isoform of GFER: Isoform 1 and isoform 2. Isoform 2 missing 1 – 80 aa. Isoform 1 is mainly located in mitochondrial intermembrane space, while Isoform 2 is secreted to cytoplasm. |
| Physical form: | Lyophilized from 0.22 μm filtered solution in 20 mM Tris, 100 mM NaCl, pH 8.0. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization. |
| Reconstitution: | Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 100 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥92% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |