IL-7 human
SIGMA/SRP6294 - recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)
Synonym: Interleukin-7
Product Type: Chemical
Immunoblotting IL-7 Cat. No. SRP6294, Human Recombinant IL-7.
| assay | ≥95% (SDS-PAGE) |
| biological source | human |
| form | lyophilized powder |
| impurities | <1 EU/μg endotoxin (LAL test) |
| mol wt | calculated mol wt 17 kDa (no tag) |
| observed mol wt 19 kDa (DTT-reduced. Protein migrates due to glycosylation. Asp26 is the predicted N-terminus.) | |
| observed mol wt 22 kDa | |
| observed mol wt 26 kDa | |
| packaging | pkg of 10 μg |
| recombinant | expressed in HEK 293 cells |
| shipped in | wet ice |
| storage temp. | −20°C |
| UniProt accession no. | P13232   |
| Biochem/physiol Actions: | Interleukin-7 (IL-7) plays a major role in innate and adaptive immunity. It participates in B and T cell development and osteoblasts differentiation of multiple myeloma. IL-7 is also involved in the development, survival and proliferation of lymphocytes. It has the ability to prevent the initiation of MAPK signaling. |
| General description: | Interleukin 7, also known as IL7, IL-7, and is a hematopoietic growth factor secreted by stromal cells in the red marrow and thymus. It is also produced by keratinocytes, dendritic cells, hepatocytes, neurons, and epithelial cells but is not produced by lymphocytes. IL-7 stimulates the differentiation of multipotent (pluripotent) hematopoietic stem cells into lymphoid progenitor cells. It also stimulates proliferation of all cells in the lymphoid lineage (B cells, T cells and NK cells). It is important for proliferation during certain stages of B-cell maturation, T and NK cell survival, development and homeostasis. IL-7 is a cytokine important for B and T cell development. This cytokine and the hepatocyte growth factor (HGF) form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. IL-7 binds to the IL-7 receptor, a heterodimer consisting of Interleukin-7 receptor α and common gamma chain receptor. Il-7 promotes hematological malignacies (acute lymphoblastic leukemia, T cell lymphoma). Elevated levels of IL-7 have also been detected in the plasma of HIV-infected patients. IL-7 as an immunotherapy agent has been examined in many pre-clinical animal studies and more recently in human clinical trials for various malignancies and during HIV infection. IL-7 could also be beneficial in improving immune recovery after allogenic stem cell transplant. |
| General description: | Interleukin-7 (IL-7) is a cytokine. It is synthesized by stromal cells, epithelial cells, endothelial and immune cells. The IL-7 gene is located on human chromosome 8q21. |
| Physical form: | Lyophilized from 0.22 μm filtered solution in in PBS, pH 7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization. |
| Reconstitution: | Centrifuge the vial prior to opening. Reconstitute in sterile deionized water to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥95% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |