IL-1 Alpha human
SIGMA/SRP6295 - recombinant, expressed in HEK 293 cells, ≥97% (SDS-PAGE)
Synonym: IL-1α; IL-1A; IL1; IL1 ALPHA; IL1-ALPHA; IL1A; IL1F1; Interleukin; Interleukin-1 alpha
Product Type: Chemical
Immunoblotting IL-1 Alpha Cat. No. SRP6295, Human Recombinant IL-1 alpha.
| assay | ≥97% (SDS-PAGE) |
| biological source | human |
| form | lyophilized powder |
| mol wt | calculated mol wt 18.0 kDa (no tag) |
| observed mol wt 22 kDa by SDS-PAGE (DTT-reduced.) | |
| packaging | pkg of 10 μg |
| recombinant | expressed in HEK 293 cells |
| shipped in | wet ice |
| storage temp. | −20°C |
| UniProt accession no. | P01583   |
| Biochem/physiol Actions: | Interleukin-1α (IL1α) possesses a wide spectrum of metabolic, physiological, hematopoietic activities, and plays one of the central roles in the regulation of the immune responses. It binds to the interleukin-1 receptor. The constitutive production of large amounts of IL-1α precursor by healthy epidermal keratinocytes interfere with the important role of IL-1α in immune responses, assuming skin as a barrier, which prevents the entry of pathogenic microorganisms into the body. In vitro, IL-1α possesses biological effect on cells in the picomolar to femtomolar range. In vivo, shortly after an onset of an infection into organism, IL-1α activates a set of immune system response processes. |
| General description: | Interleukin-1α (IL1α) is also known as IL-1A, IL1, IL1-α, IL1F1, and is a cytokine of the interleukin-1 family. IL-1α is constitutively produced by epithelial cells. It is found in substantial amounts in normal human epidermis and is distributed in a 1:1 ratio between living epidermal cells and stratum corneum. The gene encoding this protein is localized on human chromosome 2. |
| Physical form: | Lyophilized from 0.22 μm filtered solution in in PBS, pH 7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization. |
| Reconstitution: | Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥97% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |