PDGF-BB human
SIGMA/SRP6296 - recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)
Synonym: FLJ12858; PDGF-2; PDGF-B; PDGF2; PDGFB
Product Type: Chemical
Immunoblotting PDGF-BB Cat. No. SRP6296, Human Recombinant PDGF-BB.
| assay | ≥95% (SDS-PAGE) |
| biological source | human |
| form | lyophilized powder |
| mol wt | calculated mol wt 25 kDa |
| dimer observed mol wt 15 kDa (DTT-reduced. non-reduced cystine-linked homodimer) | |
| observed mol wt 30 kDa | |
| packaging | pkg of 10 μg |
| recombinant | expressed in HEK 293 cells |
| shipped in | wet ice |
| storage temp. | −20°C |
| UniProt accession no. | P01127   |
| Application: | PDGF-BB has been used as a growth factor during culturing of chondrocytes. It has been used in the differentiation of TR-BME2, an immortalized bone marrow EPC (endothelial progenitor cells)-derived cell line to vascular smooth muscle cells. |
| Biochem/physiol Actions: | PDGFs are mitogenic during early developmental stages, driving the proliferation of undifferentiated mesenchyme and some progenitor populations. During later maturation stages, PDGF signalling has been implicated in tissue remodelling and cellular differentiation, and in inductive events involved in patterning and morphogenesis. In addition to driving mesenchymal proliferation, PDGFs have been shown to direct the migration, differentiation and function of a variety of specialized mesenchymal and migratory cell types, both during development and in the adult animal. Other growth factors in this family include vascular endothelial growth factors B and C (VEGF-B, VEGF-C), which are active in angiogenesis and endothelial cell growth, and placenta growth factor (PlGF) which is also active in angiogenesis. PDGF plays a role in embryonic development, cell proliferation, cell migration, and angiogenesis. PDGF is a required element in cellular division for fibroblast, a type of connective tissue cell. PDGF is also known to maintain proliferation of oligodendrocyte progenitor cells. Mutations in this gene are associated with meningioma. |
| General description: | Platelet-derived growth factor subunit B, also known as PDGFB, FLJ12858, PDGF2, SIS, SSV, c-sis, is a member of the platelet-derived growth factor family. PDGFB can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor a polypeptide (PDGF-AB), where the dimers are connected by disulfide bonds. |
| Physical form: | Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl pH 8.0. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization. |
| Reconstitution: | Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥95% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |