LAG3/CD223 human
SIGMA/SRP6298 - recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)
Synonym: FDC
Product Type: Chemical
Immunoblotting LAG3/CD223 Cat. No. SRP6298, Human Recombinant LAG3 /CD223.
| assay | ≥95% (SDS-PAGE) |
| biological source | human |
| form | lyophilized |
| mol wt | calculated mol wt 47 kDa |
| observed mol wt 60 kDa (DTT-reduced. Protein migrates due to glycosylation. Leu 23 is the predicted N-terminus.) | |
| packaging | pkg of 10 and 50 μg |
| recombinant | expressed in HEK 293 cells |
| shipped in | wet ice |
| storage temp. | −20°C |
| tag | 6-His tagged (C-terminus) |
| UniProt accession no. | P18627   |
| Biochem/physiol Actions: | Studies in mice with null mutation for LAG3 (lymphocyte activation gene 3) gene show that this protein is involved in pathways involving natural killer (NK) cells, as in these mice, NK cells are incapable of killing certain tumor targets. This protein is expressed by activated CD4+ T cells, which results in the production of IFN-γ (interferon). This protein is thought to interact with MHC II (major histocompatibility class) molecules on immature DCs (dendritic cells), thus activating and maturing APCs (antigen presenting cells). |
| General description: | LAG3 (lymphocyte activation gene 3), first identified in activated natural killer cells (NK cells), is a transmembrane protein belonging to the Ig (immunoglobulin) superfamily. It is composed of four extracellular Ig-like domains, and the outermost domain has an unusual loop-like insertion. This cell is expressed in activated NK and T cells. |
| Physical form: | Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization. |
| Reconstitution: | Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥95% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |