Apolipoprotein A-IV from human plasma
SIGMA/SRP6301 - ≥85% (SDS-PAGE)
Synonym: Apo-AIV; ApoA-IV; Apolipoprotein A-IV; apo AIV
Product Type: Chemical
Immunoblotting Apolipoprotein AIV, human Plasma Cat. No. SRP6301, 4-12% Bis-Tris-HCI NuPAGE gel of Apolipoprotein AIV, human Plasma. Lanes 1. MW Standard 2. Apo AIV - 5 μg (reduced/heated) 3. Apo AIV - 10 μg (reduced/heated) 4. Apo AIV - 5 μg (non-reduced/no heat) 5. Apo AIV - 10 μg (non-reduced/no heat)
| assay | ≥85% (SDS-PAGE) |
| biological source | human plasma |
| form | frozen liquid |
| mol wt | 46 kDa |
| packaging | pkg of 50 μg |
| shipped in | dry ice |
| storage temp. | −70°C |
| UniProt accession no. | P06727   |
| Biochem/physiol Actions: | Apolipoprotein (apo)A-IV participates in dietary lipid and glucose metabolism. It inhibits oxidation of lipids and represses inflammation. A-IV plays a major role in changing the glucose sensitivity of pancreatic islets to alter insulin secretion. |
| General description: | Apolipoprotein (apo)A-IV4 is a lipid-binding protein. It is produced by the small intestine and liberated into the gastric lymphatics. A-IV4 belongs to the exchangeable apolipoproteins family. This gene is located on human chromosome 11q23. |
| General description: | Apolipoprotein AIV (Apo AIV) is a glycoprotein synthesized by the human intestine. Synthesis appears to be stimulated by the absorption of high density lipoproteins that are rich in triglycerides rather than by the uptake or re-esterification of fatty acids. The formation of chylomicrons acts as a signal for the induction of Apo AIV synthesis a signal enhanced by a factor from the ileum, probably PYY. Apo AIV alters the activity of the key enzymes (LPL and LCAT) of lipoprotein metabolism and cholesterol efflux from extra hepatic tissues. Also, since stimulation of intestinal synthesis and secretion by lipid absorption are rapid, Apo AIV likely plays a role in the short-term regulation of food intake. Other evidence suggests involvement in the long-term regulation of both food intake and body weight. Chronic ingestion of high-fat diet blunts the intestinal Apo AIV response to lipid feeding and, consequently, may explain why the chronic ingestion of a high-fat diet predisposes both animals and humans to obesity. It is required for efficient activation of lipoprotein lipase by ApoC-II and is a potent activator of LCAT. Apo AIV is an amphipathic protein that can emulsify lipids and has been linked to protective roles against cardiovascular disease and obesity. |
| Physical form: | Frozen in 10 mM NH4HCO3, pH 8.2. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥85% (SDS-PAGE) |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |