Apolipoprotein B from human plasma
SIGMA/SRP6302 - ≥95% (SDS-PAGE)
Synonym: Apo-B
Product Type: Chemical
Immunoblotting Apolipoprotein B, human Plasma Cat. No. SRP6302, 4-12% Bis-Tris-HCI NuPAGE gel of Apolipoprotein B, human Plasma. Lanes 1. Apo B - 5 μg (reduced/heated) 2. Apo B - 10 μg (reduced/heated) 3. Apo B - 20 μg (reduced/heated) 4. MW Standard 5. Apo B - 5 μg (non-reduced/no heat) 6. Apo B - 10 μg (non-reduced/no heat) 7. Apo B - 20 μg (non-reduced/no heat)
| assay | ≥95% (SDS-PAGE) |
| biological source | human plasma |
| form | lyophilized |
| mol wt | 550 kDa |
| packaging | pkg of 500 μg |
| shipped in | wet ice |
| storage temp. | −20°C |
| UniProt accession no. | P04114   |
| Biochem/physiol Actions: | Apo B is thought to stabilize lipid emulsions, serve as a cofactor and modulator of enzymatic reactions, manage export of lipids out of cells and direct lipids to target organs. Apo B levels are positively correlated with the risk of coronary disease. Apo B levels may be a more sensitive predictor of cardiovascular risk than LDL levels and do not involve fasting for accurate measurement. Mutations in this gene or its regulatory region cause hypoβ-lipoproteinemia, normotriglyceridemic and hypercholesterolemia due to ligand-defective ApoB, diseases affecting plasma cholesterol and ApoB levels. |
| General description: | Apolipoprotein B is the dominant protein constituent of low-density lipoprotein (LDL). The concentration of Apo B in normal plasma is 90mg/100mL. Two forms of Apo B exist: Apo B-100 and Apo B-48. The first is found in VLDL and LDL and is produced by the liver. The second is found in chylomicrons and originates in the intestine. The gene encoding this protein is localized on chromosome 2p24.1. |
| Physical form: | Lyophilized from 10 mM Na deoxycholate, pH 10.0, with 50 mM Na2CO3 and 50 mM NaCI. |
| Reconstitution: | In water or aqueous buffer |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥95% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |