Apolipoprotein E from human plasma
SIGMA/SRP6303 - ≥95% (SDS-PAGE)
Synonym: ApoE
Product Type: Chemical
Immunoblotting Apolipoprotein E, human Plasma Cat. No. SRP6303, 12% Tris-HCI SDS-PAGE Ready gel of Apolipoprotein E, human Plasma. Lanes 1. MW Standard 2. Apo E - 10 μg (reduced/no heat) 3. Apo E - 10 μg (non-reduced/no heat)
| assay | ≥95% (SDS-PAGE) |
| biological source | human plasma |
| form | frozen liquid |
| mol wt | 34 kDa |
| packaging | pkg of 50 μg |
| shipped in | dry ice |
| storage temp. | −70°C |
| UniProt accession no. | P02649   |
| Biochem/physiol Actions: | Apolipoprotein E (ApoE) serves as a ligand for low density receptors and participates in the transport and redistribution of cholesterol and other lipids. Other functions include immunoregulation, cell growth modulation and differentiation. Apo E is thought to be involved in tissue repair as increased amounts of the protein are found at sites of peripheral nerve injury and regeneration. A mutant form is associated with familial type III hyperlipoproteinemia. In addition to facilitating solubilization of lipids, these proteins help to maintain the structural integrity of lipoproteins, serve as ligands for lipoprotein receptors, and regulate the activity of enzymes involved in lipid metabolism. ApoE’s interaction with specific ApoE receptor enables uptake of chylomicron remnants by liver cells, which is an essential step during normal lipid metabolism. It also binds with the low-density lipoprotein (LDL) receptor (apo B/E). |
| General description: | Apolipoprotein E (ApoE) is an important constituent of all plasma lipoproteins. The concentration of Apo E in normal plasma is 5 mg/100mL. Significant quantities of ApoE are produced in liver and brain and to some extent in almost every organ. It exists in three major isoforms; E2, E3, and E4, which differ from one another by a single amino-acid substitution. The gene encoding this protein is localized on human chromosome 19q13.32. |
| Physical form: | Frozen in 50 mM NH4HCO3, pH 8.0. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥95% (SDS-PAGE) |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |