Alpha 1 Antitrypsin from human plasma
SIGMA/SRP6312 - HBsAg, HCV, HBc free, ≥95% (SDS-PAGE)
Synonym: AAT; Alpha-1 protease inhibitor; Alpha-
Product Type: Chemical
Immunoblotting Alpha 1 Antitrypsin,human Plasma Cat. No. SRP6312, 4-12% Bis-Tris NuPAGE gel of Alpha 1 Antitrypsin,human Plasma. Lanes 1. A1P1 - 5 μg (reduced/heated) 2. A1P1 - 10 μg (reduced/heated) 3. A1P1 - 20 μg (reduced/heated) 4. LMW Standard 5. A1P1 - 5 μg (non-reduced/no heat) 6. A1P1 - 10 μg (non-reduced/no heat) 7. A1P1 - 20 μg (non-reduced/no heat)
| assay | ≥95% (SDS-PAGE) |
| biological source | human |
| form | lyophilized |
| mol wt | 52 kDa |
| packaging | pkg of 1 mg |
| potency | ≥5.0 I.U. per mg |
| shipped in | wet ice |
| storage temp. | −20°C |
| UniProt accession no. | P01009   |
| Application: | AAT (α 1 antitrypsin) from human plasma has been used as a standard to check the presence of AAT in benign and malignant lymphoreticular cells and tissues. |
| Biochem/physiol Actions: | AAT (α 1 antitrypsin) functions as a serine protease inhibitor with targets like elastase, plasmin, collagenase, thrombin, leucocyte proteases, trypsin, chymotrypsin and plasminogen activator. Clinically, its deficiency is associated with two major diseases: pulmonary emphysema and early onset/juvenile hepatic cirrhosis. It is elevated in inflammatory conditions, malignancies, liver disease and pregnancy and also after surgical trauma and use of oral contraceptives. The simultaneous quantitative determination of AAT and ceruloplasmin permits differential diagnosis of liver afflictions. Rise in AAT occurs as an acute phase response to tissue necrosis and inflammation. It might work as a biomarker for cutaneous squamous cell carcinoma, hepatitis B, insulinomas, NSCLC (non-small cell lung cancer), papillary thyroid carcinoma, lung cancer and breast carcinoma. |
| General description: | Our Alpha 1 Antitrypsin is a human plasma protein.It is shown to be HBsAg, HCV, HBc free and also negative for anti HIV1 and HIV2 according to FDA approved tests. The gene AAT (α 1 antitrypsin) is mapped to human chromosome 14q32.1. AAT is an acute-phase plasma protein. It is a water soluble glycoprotein. AAT is mainly secreted by hepatocytes, macrophages, and some cancer cells. AAT is also referred to as Serpin A1. |
| Physical form: | Lyophilized from 30 mM Na Phosphate, pH 6.5 with 300 mM NaCl. |
| Reconstitution: | In water or aqueous buffer |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Purity | ≥95% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |