Kininogen,LMW from human plasma
SIGMA/SRP6319 - ≥95% (SDS-PAGE)
Synonym: Alpha-2-thiol proteinase inhibitor; BDK; Fitzgerald factor; High molecular weight Kininogen; KNG; Kininogen-1 heavy chain; Low molecular weight growth-promoting; Williams-
Product Type: Chemical
Immunoblotting Kininogen LMW, human Plasma Cat. No. SRP6319, 12% Tris Glycine gel of Kininogen LMW, human Plasma. Lanes 1. LMW Standard 2. Kininogen, LMW - 5 μg (reduced/heated) 3. Kininogen, LMW - 10 μg (reduced/heated) 4. Kininogen, LMW - 20 μg (reduced/heated) 5. Kininogen, LMW - 5 μg (non-reduced/no heat) 6. Kininogen, LMW - 10 μg (non-reduced/no heat) 7. Kininogen, LMW - 20 μg (non-reduced/no heat)
| assay | ≥95% (SDS-PAGE) |
| biological source | human plasma |
| form | lyophilized |
| mol wt | 65 kDa |
| packaging | pkg of 100 μg |
| shipped in | wet ice |
| storage temp. | −20°C |
| UniProt accession no. | P01042   |
| Biochem/physiol Actions: | KNG1 (kininogen 1) exists in low and high molecular weight forms which are precursors for bradykinin and kallidin (Lys-Bradykinin), respectively. The high molecular weight kininogen (HK) functions as a cofactor for the stimulation of surface-bound Hageman factor (Factor XII) by kallikrein. HK is subsequently required for the activation of prekallikrein and Factor XI by the activated Hageman factor. These steps are essential for the initiation of coagulation, fibrinolysis, and the production of vasoactive peptides. Ile581Thr polymorphism in KNG1 gene is linked with increased susceptibility to venous thrombosis. |
| General description: | KNG1 (kininogen 1) gene is localized to human chromosome 3, and codes for both high and low molecular weight kininogen. The high molecular weight kininogen (HK) is a 120kDa single chain glycoprotein. |
| General description: | LMW Kininogen is involved in blood pressure regulation since it is an endogenous protein substrate for tissue kallikrein. Proteolytic cleavage by kallikrein releases vasoactive bradykinin from LMW kininogen. In addition, LMW kininogen, like HMW kininogen, is a major extracellular cysteine protease inhibitor. |
| Physical form: | Lyophilized from 10 mM Na acetate, pH 5.5 with 200 mM NaCI . |
| Reconstitution: | In water or aqueous buffer |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥95% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |