CD274 from mouse
SIGMA/SRP6327 - recombinant, expressed in E. coli, ≥85% (SDS-PAGE)
Synonym: A530045L16Rik; B7h1; CD274 antigen; Pdcd1l1; Pdcd1lg1; Pdl1
Product Type: Chemical
Immunoblotting CD274, mouse Cat. No. SRP6327, 15% SDS PAGE (3 μg) of mouse recombinant CD274.
| assay | ≥85% (SDS-PAGE) |
| biological source | mouse |
| concentration | 1 mg/mL |
| form | liquid |
| impurities | <1 EU/μg endotoxin (LAL test) |
| mol wt | 27.6 kDa (246 aa, 19-239 aa + His tag) |
| packaging | pkg of 100 μg |
| recombinant | expressed in E. coli |
| shipped in | dry ice |
| storage temp. | −70°C |
| UniProt accession no. | Q9EP73   |
| Biochem/physiol Actions: | Programmed cell death 1 ligand 1 (PD-L1) is involved in the regulation of cellular and humoral immune responses. PD-L1 is up-regulated on T- and B-cells, dendritic cells, keratinocytes and monocytes after lipopolysaccharide (LPS) and interferon-γ (IFNG) activation and up-regulated in B-cells activated by surface Ig cross-linking. PD-L1 is involved in the co-stimulatory signal, essential for T-cell proliferation and production of interleukin-10 (IL10) and IFNG, in an interleukin-2-dependent and a programmed cell death 1 (PDCD1)-independent manner. |
| General description: | Programmed cell death 1 ligand 1 (PD-L1), which is also known as cluster of differentiation (CD274) or B7 homolog 1 (B7-H1), is a member of the growing B7 family of immune molecules. PD-L1 is a cell surface immunoglobulin with two Ig-like domains within the extracellular region and a short cytoplasmic domain. The protein is highly expressed in the heart, skeletal muscle, placenta and lung and weakly expressed in the thymus, spleen, kidney and liver. PD-L1 is expressed on activated T-cells, B-cells, dendritic cells, keratinocytes and monocytes. Recombinant mouse CD274 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography. |
| Physical form: | 1 mg/mL in 20 mM Tris-HCl buffer (pH 8.0) containing 0.15 M NaCl and 10% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥85% (SDS-PAGE) |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |