CD3E human
SIGMA/SRP6354 - recombinant, expressed in E. coli, ≥90% (SDS-PAGE)
Synonym: T-cell surface glycoprotein CD3 epsilon chain; T3E; TCRE
Product Type: Chemical
Immunoblotting CD3E Cat. No. SRP6354, 15% SDS PAGE (3 μg) of human recombinant CD3E.
| assay | ≥90% (SDS-PAGE) |
| biological source | human |
| concentration | 1 mg/mL |
| form | liquid |
| mol wt | 14.1 kDa (127 aa, 23-126 aa + His Tag) |
| packaging | pkg of 50 μg |
| recombinant | expressed in E. coli |
| shipped in | dry ice |
| storage temp. | −70°C |
| UniProt accession no. | P07766   |
| General description: | CD3E is the CD3-epsilonpolypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor α/β and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women. Recombinant human CD3E protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
| Physical form: | 1 mg/mL in 20 mM Tris-HCl buffer (pH 8.0) containing 0.2 M NaCl, and 10% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥90% (SDS-PAGE) |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |