Aldose Reductase human
SIGMA/SRP6371 - recombinant, expressed in E. coli, ≥95% (SDS-PAGE)
Synonym: ADR; AKR1B1; ALDR1; ALR2; AR; Aldo-keto reductase family1; member B1
Product Type: Chemical
Immunoblotting Aldose Reductase Cat. No. SRP6371, 15% SDS PAGE (3 μg) of human recombinant Aldose Reductase.
| assay | ≥95% (SDS-PAGE) |
| biological source | human |
| concentration | 1 mg/mL |
| form | liquid |
| mol wt | 35.8 kDa (316 aa, 1-316 aa) |
| packaging | pkg of 100 μg |
| recombinant | expressed in E. coli |
| shipped in | dry ice |
| storage temp. | −70°C |
| UniProt accession no. | P15121   |
| Biochem/physiol Actions: | Aldose reductase (AKR1B1) catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols. This protein is implicated in the development of diabetic complications by catalyzing the reduction of glucose to sorbitol. AKR1B1 is also involved in cytokine-mediated generation of prostaglandin F2α (PGF2α) in preadipocytes. It participates in diabetes-associated mitochondrial disorder and damage via the activation of p53. AKR1B1 is associated with drug resistance mechanism in cancer-derived cell lines. It is also known to mediate cellular processes linked to carcinogenesis such as EMT (epithelial–mesenchymal transition), inflammation and angiogenesis. Aldose reductase mediates glucose metabolism in placenta and osmotic control in eye and kidney. |
| General description: | Aldose reductase (AKR1B1) is a member of the aldo-keto reductase (AKR) superfamily. Recombinant aldose reductase (AKR1B) protein was expressed in Escherichia coli and purified by using conventional chromatography techniques. It is expressed in eye and kidney, but predominant in placenta. The AKR1B1 gene is mapped to human chromosome 7q33. |
| Physical form: | 1 mg/mL in 20 mM Tris-HCl buffer (pH 8.0) containing 1 mM DTT and 10% glycerol. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H319 |
| Precautionary statements | P264 - P280 - P305 + P351 + P338 - P337 + P313 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥95% (SDS-PAGE) |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |