Cytidine deaminase human
SIGMA/SRP6372 - recombinant, expressed in E. coli, ≥90% (SDS-PAGE)
Synonym: CDA; CDD; Cytidine aminohydrolase; Cytosine nucleoside deaminase
Product Type: Chemical
assay | ≥90% (SDS-PAGE) |
biological source | human |
concentration | 0.5 mg/mL |
form | liquid |
mol wt | 18.3 kDa (166 aa, 1-146 aa + His Tag.) |
packaging | pkg of 100 μg |
recombinant | expressed in E. coli |
shipped in | dry ice |
storage temp. | −70°C |
UniProt accession no. | P32320 |
Biochem/physiol Actions: | Cytidine deaminase (CDA) is mainly expressed in granulocytes and can form homotetramers. Recombinant human CDA protein, fused to His-tag at N-terminus, was expressed in Escherichia coli and purified by using conventional chromatography. |
General description: | Cytidine deaminase (CDA) is an enzyme that scavenges exogenous and endogenous cytidine and 2′-deoxycytidine for UMP synthesis. This protein is one of several deaminases responsible for maintaining the cellular pyrimidine pool. CDA also catalyzes the deamination of chemotherapeutic cytosine nucleoside analogs such as arabinofuranosyl cytidine (Ara-C) and 5-azacytidine, which results in the loss of their cytotoxic and antitumor function. |
Physical form: | 0.5 mg/mL in 20 mM Tris-HCl buffer (pH 8.0) containing 100 mM NaCl, 1 mM DTT, 2 mM EDTA and 40% glycerol. |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 3 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Purity | ≥90% (SDS-PAGE) |
Storage Temp. | −70°C |
UNSPSC | 12352200 |