PYCR1 human
SIGMA/SRP6375 - recombinant, expressed in E. coli, ≥90% (SDS-PAGE)
Synonym: ARCL2B; P5C; P5CR; PIG45; PP222; PRO3; Pyrroline-
Product Type: Chemical
Immunoblotting PYCR1 Cat. No. SRP6375, 15% SDS PAGE (3 μg) of human recombinant PYCR1.
| assay | ≥90% (SDS-PAGE) |
| biological source | human |
| concentration | 0.5 mg/mL |
| form | liquid |
| mol wt | 35.5 kDa (339 aa, 1-319 aa + His Tag.) |
| packaging | pkg of 50 μg |
| recombinant | expressed in E. coli |
| shipped in | dry ice |
| storage temp. | −70°C |
| UniProt accession no. | P32322   |
| General description: | PYCR1 is a universal housekeeping enzyme that catalyzes the NAD (P) H-dependent conversion of pyrroline-5-carboxylate to proline. This enzyme may also play a physiologic role in the generation of NADP (+) in some cell types. It forms a homopolymer and localizes to the mitochondrion. Defects in PYCR1 are the cause of cutis laxa autosomal recessive type 2B (ARCL2B). Recombinant human PYCR1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography. |
| Physical form: | 0.5 mg/mL in 20 mM Tris-HCl buffer (pH 8.5) containing 10% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥90% (SDS-PAGE) |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |