CD273 human
SIGMA/SRP6376 - recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)
Synonym: Butyrophilin B7-DC; PDCD1 ligand 2; PDCD1L2; PDCD1LG2; PDL2
Product Type: Chemical
assay | ≥95% (SDS-PAGE) |
biological source | human |
form | lyophilized |
mol wt | calculated mol wt 23.4 kDa |
observed mol wt 35-48 kDa (DTT-reduced. Protein migrates due to glycosylation. Leu 20 is the predicted N-terminus.) | |
packaging | pkg of 10 μg |
pkg of 50 μg | |
recombinant | expressed in HEK 293 cells |
shipped in | wet ice |
storage temp. | −20°C |
tag | 6-His tagged (C-terminus) |
UniProt accession no. | Q9BQ51 |
Biochem/physiol Actions: | The expression of programmed cell death 1 ligand 2 (PD-L2) or CD antigen 273 (CD273) is up-regulated by interferon-γ (IFNG/IFN-γ) stimulation in monocytes and induced on dendritic cells grown from peripheral blood mononuclear cells with colony-stimulating factor-2 (CSF2) and interleukin-4 (IL-4). PD-L2 is involved in the costimulatory signal, essential for T-cell proliferation and IFNG production in a PDCD1-independent manner. PD-L2 interaction with PDCD1 inhibits T-cell proliferation by blocking cell cycle progression and cytokine production. |
General description: | Programmed cell death 1 ligand 2 (PD-L2 or PDCD1 ligand 2) is also known as butyrophilin B7-DC or CD antigen CD273. It belongs to the immunoglobulin superfamily or BTN/MOG family. The gene encoding CD273 is localized on human chromosome 9p24.1. |
Physical form: | Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization. |
Reconstitution: | Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C. |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 3 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Purity | ≥95% (SDS-PAGE) |
Storage Temp. | −20°C |
UNSPSC | 12352200 |