CD319/SLAMF7/CRACC human
SIGMA/SRP6383 - recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)
Synonym: 19A; CS1; FOAP-12
Product Type: Chemical
Immunoblotting CD319 / SLAMF7 / CRACC Cat. No. SRP6383, SDS PAGE of human recombinant CD319/SLAMF7/CRACC.
| assay | ≥95% (SDS-PAGE) |
| biological source | human |
| form | lyophilized |
| impurities | <1 EU/μg endotoxin (LAL test) |
| mol wt | calculated mol wt 23.2 kDa |
| observed mol wt 33-48 kDa (DTT-reduced. Protein migrates due to glycosylation. Ser 23 is the predicted N-terminus.) | |
| packaging | pkg of 10 μg |
| pkg of 50 μg | |
| recombinant | expressed in HEK 293 cells |
| shipped in | wet ice |
| storage temp. | −20°C |
| tag | 6-His tagged (C-terminus) |
| UniProt accession no. | Q9NQ25   |
| Biochem/physiol Actions: | SLAMF7 (signaling lymphocytic activation molecule F7) is considered as a self-ligand which identifies another SLAMF7 expressed on the cell. Apart from immune cells, SLAMF7 is highly expressed on multiple myeloma cells. |
| General description: | SLAM family member 7 (SLAMF7) is also known as CD2-like receptor-activating cytotoxic cells (CRACC), membrane protein FOAP-12, CD antigen CD319, Novel Ly9, Protein 19A, which is a single-pass type I membrane protein and a member of the CD2 family of cell surface receptors. SLAMF7 is expressed in spleen, lymph node, peripheral blood leukocytes, bone marrow, small intestine, stomach, appendix, lung and trachea. Isoform 1 of SLAMF7 mediates natural killer (NK) cell activation through a SH2 domain containing 1A (SH2D1A)-independent extracellular signal-regulated extracellular signal-regulated kinases (ERK) mediated pathway. It may play a role in lymphocyte adhesion. Isoform 3 of SLAMF7 does not mediate any NK cell activation. SLAM F7 (signaling lymphocytic activation molecule F7) receptors are expressed on immune cells such as activated T cells, most B cells, antibody-producing plasma cells and natural killer cell and also on myeloid cells. The SLAMF7 gene is mapped to human chromosome 1q23.3. |
| Physical form: | Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization. |
| Reconstitution: | Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥95% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |