BRD3 (1-416) his tag human
SIGMA/SRP6413 - recombinant, expressed in E. coli, ≥85% (SDS-PAGE)
Synonym: Bromodomain containing protein 3; ORFX; RING3-like protein; RING3L
Product Type: Chemical
assay | ≥85% (SDS-PAGE) |
biological source | human |
concentration | 0.5 mg/mL |
form | liquid |
mol wt | 48.1 kDa (439 aa, 1- 416 aa + His Tag) |
NCBI accession no. | NM_007371 |
packaging | pkg of 100 μg |
recombinant | expressed in E. coli |
shipped in | dry ice |
storage temp. | −70°C |
UniProt accession no. | Q15059 |
General description: | The acetylation of histone lysine residues plays a crucial role in the epigenetic regulation of gene transcription. A bromodomain is a protein domain that recognizes acetylated lysine residues such as those on the N-terminal tails of histones. This recognition is often a prerequisite for protein-histone association and chromatin remodeling. These domains function in the linking of protein complexes to acetylated nucleosomes, thereby controlling chromatin structure and gene expression. Thus, bromodomains serve as “readers” of histone acetylation marks regulating the transcription of target promoters. The BET family of proteins, defined by tandem Bromodomains and an Extra Terminal domain, include BRD2, BRD3, BRD4, and BRDT. The BET proteins play a key role in many cellular processes, including inflammatory gene expression, mitosis, and viral/host interactions. The isolated individual or tandem bromodomains of BRD3 have been shown to bind acetylated histone tails, serving to couple histone acetylation marks to the transcriptional regulation of target promoters. Small molecule inhibitors of these interactions hold promise as useful therapeutics for human disease. Recombinant human BRD3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
Physical form: | 0.5 mg/mL in Phosphate buffer saline (pH 7.4) containing 10% glycerol. |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 3 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Purity | ≥85% (SDS-PAGE) |
Storage Temp. | −70°C |
UNSPSC | 12352200 |