Kallikrein-1 human
SIGMA/SRP6420 - recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)
Synonym: KLK1; KLKR; Klk6; hK1
Product Type: Chemical
Immunoblotting Kallikrein-1 Cat. No. SRP6420, SDS PAGE of human recombinant Kallikrein-1.
| assay | ≥95% (SDS-PAGE) |
| biological source | human |
| form | lyophilized |
| mol wt | calculated mol wt 27.9 kDa |
| observed mol wt 40-45 kDa (DTT-reduced. Protein migrates due to glycosylation. Pro 19 is the predicted N-terminal.) | |
| packaging | pkg of 10 μg |
| recombinant | expressed in HEK 293 cells |
| shipped in | wet ice |
| storage temp. | −20°C |
| tag | 6-His tagged (C-terminus) |
| UniProt accession no. | P06870   |
| General description: | Kallikrein-1 (KLK1) belongs to the peptidase S1 family and Kallikrein subfamily. KLK1 contains one peptidase S1 domain. KLK-1 preferential cleavage of Arg-|-Xaa bonds in small molecule substrates and also has highly selective action to release kallidin (lysyl-bradykinin) from kininogen involves hydrolysis of Met-|-Xaa or Leu-|-Xaa. Glandular Kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin. |
| Physical form: | Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 2 mM CaCl2, 150 mM NaCl, pH 7.5. Normally Mannitol or Trehalose is added as protectants before lyophilization. |
| Reconstitution: | Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥95% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |