ICOSLG/B7-H2/CD275 human
SIGMA/SRP6431 - recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)
Synonym: B7H2; B7RP-1; B7RP1; GL50; ICOS ligand; ICOS-L; ICOSL; LICOS
Product Type: Chemical
Immunoblotting ICOSLG / B7-H2 / CD275 Cat. No. SRP6431, SDS PAGE of human recombinant ICOSLG/B7-H2/CD275.
| assay | ≥95% (SDS-PAGE) |
| biological source | human |
| form | lyophilized |
| impurities | <1 EU/μg |
| mol wt | calculated mol wt 27.5 kDa |
| observed mol wt 45-50 kDa (DTT-reduced. Protein migrates due to glycosylation. Asp 19 is the predicted N-terminal.) | |
| packaging | pkg of 20 and 100 μg |
| potency | 3.2-400 ng/mL |
| recombinant | expressed in HEK 293 cells |
| shipped in | wet ice |
| storage temp. | −20°C |
| tag | 6-His tagged (C-terminus) |
| UniProt accession no. | O75144   |
| General description: | ICOS ligand (ICOSLG) is also known as B7 homolog 2 (B7-H2), B7-related protein 1 (B7RP-1) and CD antigen CD275, which belongs to the immunoglobulin superfamily and BTN/MOG family. ICOSLG contains one Ig-like C2-type (immunoglobulin-like) domain and one Ig-like V-type (immunoglobulin-like) domain. Isoform 1 is widely expressed, while isoform 2 is detected only in lymph nodes, leukocytes and spleen. B7-H2 is ligand for the T-cell-specific cell surface receptor ICOS. B7-H2 acts as a costimulatory signal for T-cell proliferation and cytokine secretion and induces also B-cell proliferation and differentiation into plasma cells. B7-H2 could play an important role in mediating local tissue responses to inflammatory conditions, as well as in modulating the secondary immune response by co-stimulating memory T-cell function. |
| Physical form: | Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization. |
| Reconstitution: | Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥95% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |