CD58/LFA-3 human
SIGMA/SRP6432 - recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)
Synonym: Ag3
Product Type: Chemical
assay | ≥95% (SDS-PAGE) |
biological source | human |
form | lyophilized |
mol wt | calculated mol wt 22.3 kDa |
observed mol wt 30-45 kDa (DTT-reduced. Protein migrates due to glycosylation. Phe 29 is the predicted N-terminal.) | |
packaging | pkg of 10 μg |
pkg of 50 μg | |
recombinant | expressed in HEK 293 cells |
shipped in | wet ice |
storage temp. | −20°C |
tag | 6-His tagged (C-terminus) |
UniProt accession no. | P19256 |
Biochem/physiol Actions: | Cluster of differentiation 58 (CD58)/lymphocyte function-associated antigen 3 (LFA-3) is a ligand of the T-lymphocyte cluster of differentiation 2 (CD2) glycoprotein, which binds to CD2 (LFA-2) on T cells. It is important in strengthening the adhesion between the T cells and professional antigen presenting cells. In addition, the LFA-3/CD2 interaction may prime response by both the CD2+ and LFA-3+ cells. Polymorphisms in the CD58 gene are associated with increased risk for multiple sclerosis. |
General description: | Cluster of differentiation 58 (CD58), also known as lymphocyte function-associated antigen 3 (LFA-3), is a cell adhesion molecule expressed on antigen presenting cells (APC), particularly macrophages. The gene encoding this protein is localized on human chromosome 1p13.1. CD58 /LFA3 is ligand of the T-lymphocyte CD2 glycoprotein, which binds to CD2 (LFA-2) on T cells and is important in strengthening the adhesion between the T cells and Professional Antigen Presenting Cells. In addition, the LFA-3/CD2 interaction may prime response by both the CD2+ and LFA-3+ cells. Polymorphisms in the CD58 gene are associated with increased risk for multiple sclerosis. |
Physical form: | Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization. |
Reconstitution: | Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C. |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 3 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Purity | ≥95% (SDS-PAGE) |
Storage Temp. | −20°C |
UNSPSC | 12352200 |